Controlling the activity of quorum sensing autoinducers with light† †Electronic supplementary information (ESI) available: Detailed experimental information on chemical synthesis, photoswitching experiments, bacterial growth conditions, bioluminescence assay, RNA isolation and cDNA synthesis, gene expression and pyocyanine assay details. See DOI: 10.1039/c5sc00215j

نویسندگان

  • J. P. Van der Berg
  • W. A. Velema
  • W. Szymanski
  • A. J. M. Driessen
  • B. L. Feringa
چکیده

Title: Controlling the Activity of Quorum Sensing Autoinducers with Light Jan Pieter van der Berg,a§ Willem A. Velema,b§ Wiktor Szymanski,b,c Arnold J. M. Driessena* and Ben L. Feringab* aMolecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, Nijenborgh 7, 9747 AG, Groningen, The Netherlands bCentre for Systems Chemistry, Stratingh Institute for Chemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands. cDepartment of Radiology, University of Groningen, University Medical Centre Groningen, Groningen, The Netherlands. §These authors contributed equally to the manuscript.

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منابع مشابه

Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography† †Electronic supplementary information (ESI) available: Experimental procedures for microarray fabrication, deprotection and cleavage as well as LC-MS conditions and spectra of all array eluates. See DOI: 10.1039/c4cc05771f Click here for additional data file.

Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries. We describe the use of LC-MS to analyse DNA and RNA oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using ligh...

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عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2015